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One sample
A batch
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.
Well volume (in mL)
Typical value for 96-well plate is 0.1 mL.
Starting dilution
Must be ≤ 1 (e.g. 10-fold as 0.1, 4-fold as 0.25).
Dilution factor
Must be < 1 (e.g. 10-fold as 0.1, 4-fold as 0.25).
# dilutions
Typically 7 or 8 or 11 or 12.
# repeats/dilution
Typically 4 or 6 or 8.